Hyalose, LLC is a biotechnology company focused on the commercialization of unique recombinant technologies for producing Hyaluronic Acid (HA), an important biomolecule for many healthcare and cosmetic applications.
With a strong intellectual property portfolio and scientific expertise, Hyalose meets today’s market demands for highly controlled HA production.
About Us
Hyalose was founded in 2000 to address the commercial and research need for the supply of hyaluronic acid products. This unique and proprietary recombinant technology was developed by Chief Scientists Dr. Paul Weigel and Dr. Paul DeAngelis.
Hyalose is a portfolio company of leading life sciences technology management firm Emergent Technologies, Inc. with business operations based in Austin, TX.
In 2019, Hyalose selected Echelon Biosciences, Inc. of Salt Lake City, Utah, to serve as the new, sole distributor of its Select-HA products. See press release here.
Hyalose is a portfolio company of leading life sciences technology management firm Emergent Technologies, Inc. with business operations based in Austin, TX.
In 2019, Hyalose selected Echelon Biosciences, Inc. of Salt Lake City, Utah, to serve as the new, sole distributor of its Select-HA products. See press release here.
Technology
The Hyalose technologies enable the bulk production of hyaluronic acid through fermentation, as well as the controlled enzymatic synthesis that enables an exact determination of molecule output. The controlled synthesis also enables the placement of other glycosaminoglycan sugars and unnatural sugars at precisely defined positions in the sugar chain, creating new and novel sugar compounds.
Hyaluronan (hyaluronic acid, HA) is a type of sugar polymer called a polysaccharide (other common polysaccharides include cellulose from wood, or starch from food). HA is a member of the glycosaminoglycan family. The chemical structure of HA is a chain of two different simple sugars (N-acetylglucosamine and glucuronic acid) repeated over and over. In nature, the length of the polymer chains ranges from hundreds to thousand of sugars units and can exist in as short or long chains.
Hyaluronan (hyaluronic acid, HA) is a type of sugar polymer called a polysaccharide (other common polysaccharides include cellulose from wood, or starch from food). HA is a member of the glycosaminoglycan family. The chemical structure of HA is a chain of two different simple sugars (N-acetylglucosamine and glucuronic acid) repeated over and over. In nature, the length of the polymer chains ranges from hundreds to thousand of sugars units and can exist in as short or long chains.
Select-HA™
Hyalose has methods for producing HA of defined size ranges, marketed as Select-HA. These products are made through enzymatic synthesis
where a high level of size control is possible. Polydispersity of the products in any size from 50kDa to 1000kDa is as low as 1.02 and averages 1.1 (a value of 1 is associated with a ‘perfect’ polymer). Hyalose offers one grade of Select-HA products and certifies its products as having endotoxin levels of less than 0.1 EU/mg of HA polymer.
In the agarose gel (left lane), Select-HA products are tight bands, much as the DNA ladder in the middle lane. The sizes (masses) of the Select-HA products have been determined by GPC-MALLS. Other commercial HA prodcuts have much greater size heterogeneity and run as a smear (right lane) averaging 587 kDa.
Hyalose is seeking collaborators to implement this patented technology to produce products where the specific molecular weight of the HA matters.Select-HA Ladders™
Leveraging the ability to create select sizes of HA, Hyalose offers Select-HA ladders for identification of glycosaminoglycans via gel electrophoresis.
Bulk HA™
Bulk HA was traditionally sourced from animal byproducts, such as rooster comb, or bacterial strains derived from
pathogenic organisms. This traditional supply can present source dependent fluctuations of quality that may inhibit research or other end-use applications.
In May 2002, Hyalose licensed its recombinant Bulk HA technology to Novozymes Biopolymer A/S, a wholly owned subsidiary of Novozymes A/S (NYSE: NVZMF). Based on this unique expression technology Novozymes Biopharma today manufactures and markets a better defined, controlled and safe sodium hyaluronate under the brand name Hyasis.
Please see the link and brochure below for more information on Hyasis®.
Link to website
Link to brochure
In May 2002, Hyalose licensed its recombinant Bulk HA technology to Novozymes Biopolymer A/S, a wholly owned subsidiary of Novozymes A/S (NYSE: NVZMF). Based on this unique expression technology Novozymes Biopharma today manufactures and markets a better defined, controlled and safe sodium hyaluronate under the brand name Hyasis.
Please see the link and brochure below for more information on Hyasis®.
Link to website
Link to brochure
Polymer Grafting
Another significant development area for Hyalose is polymer grafting for the formation of novel carbohydrates,
including hybrid polysaccharides and HA-conjugates.
Hyalose has formed a "Tool Kit" of methods for the innovative synthesis of designer polysaccharide products utilizing recombinant enzymes. The enabling technology is the use of soluble enzymes to create unique new hybrid polymers and polysaccharide conjugates.
Hyalose is seeking collaborators to implement this patented technology to produce products where the specific molecular weight and the composition of hyaluronic acid and/or hybrids of GAGs matter.
Hyalose has formed a "Tool Kit" of methods for the innovative synthesis of designer polysaccharide products utilizing recombinant enzymes. The enabling technology is the use of soluble enzymes to create unique new hybrid polymers and polysaccharide conjugates.
Hyalose is seeking collaborators to implement this patented technology to produce products where the specific molecular weight and the composition of hyaluronic acid and/or hybrids of GAGs matter.
Advantages
Most HA is produced by extraction, either from animal by-products or from bacteria. These must be purified to obtain the desired material for a particular application. During the extraction procedures, care must be taken not to co-purify pathogens or detrimental factors commonly found in the source material (e.g., prions, antigens, allergens, or bacterial toxins). Hyalose innovation includes superior processes to produce:
Hyalose Frequently Asked Questions
- HA of a defined polymer length and content and
- HA from a Generally Regarded As Safe (GRAS) bacterial source.
Hyalose Frequently Asked Questions
Products
Select-HA™ - Select-HA is hyaluronic acid produced through enzymatic synthesis achieving a high level of size control. Polydispersity of the products in any size from 50kDa to 1000kDa is as low as 1.02 and averages 1.1 (a value of 1 is associated with a ‘perfect’ polymer). Hyalose offers one grade of Select-HA products and certifies its products as having endotoxin levels of less than 0.1 EU/mg of HA polymer.
Select-HA MegaLadder™ - The Select-HA MegaLadder is a mixture of streptavidin complexes containing one, two, three or four end-labeled biotin-Select-HA molecules of very defined sizes for use as size standards in gel electrophoresis or other separation methods. This ladder covers a range from 2 MegaDalton to 8 MegaDalton. Recommended usage: agarose gel stained with StainsAll.
Select-HA HiLadder™ - The Select-HA HiLadder contains five Select-HA molecular mass markers in the range of ~500 kDa to ~1500kDa. The masses are 495 kDa, 572 kDa, 966 kDa, 1090 kDa and 1510 kDa. Recommended usage: agarose gel stained with StainsAll.
Select-HA LoLadder™ - The Select-HA LoLadder contains five Select-HA molecular mass markers in the range of ~25 kDa to ~500 kDa. The masses are 27 kDa, 110 kDa, 214 kDa, 310 kDa and 495 kDa. Recommended usage: agarose gel stained with StainsAll.
Product ListProduct FAQs
Orders
Hyalose has partnered with Echelon Biosciences, Inc., a leading supplier in novel biochemical reagents, assays, and screening services, as the sole distributor of its products worldwide. For Select-HA product information or to place an order visit: http://echelon-inc.com/ha/
For all other inquiries, please contact us at info@hyalose.com.
For all other inquiries, please contact us at info@hyalose.com.
Publications
Brown, S. H. and Pummill, P. E. (2008) Recombinant Production of Hyaluronic Acid. Curr. Pharm. Biotechnol. 9:239-41.
Weigel, P.H. and DeAngelis P.L. (2007) Hyaluronan Synthases: A Decade-plus of Novel Glycosyltransferases. J. Biol. Chem., 282: 36777-36781.
Jing, W., Haller, M., Almond, A. and DeAngelis, P.L. (2006) Defined megadalton hyaluronan polymer standards. Anal Biochem.15;355(2):183-8.
Widner, W., Behr, R., Von Dollen, S., Tang, M., Heu, T., Sloma, A., Sternberg, D., DeAngelis, P.L., Weigel, P.H. and Brown, S. (2005) Hyaluronic acid production in Bacillus subtilis. Applied and Environmental Microbiol. 71:3747-3752.
Jing, W and DeAngelis, P.L. (2004) Synchronized chemoenzymatic synthesis of monodisperse hyaluronan polymers. J. Biol. Chem., 279(40), 42345-42349.
Weigel, P.H. and DeAngelis P.L. (2007) Hyaluronan Synthases: A Decade-plus of Novel Glycosyltransferases. J. Biol. Chem., 282: 36777-36781.
Jing, W., Haller, M., Almond, A. and DeAngelis, P.L. (2006) Defined megadalton hyaluronan polymer standards. Anal Biochem.15;355(2):183-8.
Widner, W., Behr, R., Von Dollen, S., Tang, M., Heu, T., Sloma, A., Sternberg, D., DeAngelis, P.L., Weigel, P.H. and Brown, S. (2005) Hyaluronic acid production in Bacillus subtilis. Applied and Environmental Microbiol. 71:3747-3752.
Jing, W and DeAngelis, P.L. (2004) Synchronized chemoenzymatic synthesis of monodisperse hyaluronan polymers. J. Biol. Chem., 279(40), 42345-42349.
Contact Us
Hyalose, LLC
Product Orders:
Echelon Biosciences, Inc.675 Arapeen Dr. Ste. 302
Salt Lake City, UT 84108
Phone: (801) 588-0455
Email: echelon@echelon-inc.com
Fax: (801) 588-0497
Business Operations:
Emergent Technologies, Inc.7500 Rialto Blvd., Building 2, Suite 260
Austin, Texas 78735
Phone: (512) 263-3232, ext. 8
Email: info@etibio.com
Fax: (512) 263-3236